Back to Search
Start Over
A link between the interleukin-6/Stat3 anti-apoptotic pathway and microRNA-21 in preimplantation mouse embryos.
- Source :
-
Molecular reproduction and development [Mol Reprod Dev] 2009 Sep; Vol. 76 (9), pp. 854-62. - Publication Year :
- 2009
-
Abstract
- Signal transducers and activators of transcription-3 (Stat3) plays a central role in interleukin-6 (IL-6)-mediated cell proliferation by inhibiting apoptosis in a variety of cell types. MicroRNA-21 (miRNA-21), a ubiquitous miRNA, acts as an anti-apoptotic factor that seems to be indirectly but strictly linked to Stat3. In order to determine whether the IL-6 induced Stat3 anti-apoptosis pathway is linked with miRNA-21, we first determined the effects of recombinant mouse IL-6 on Stat3 expression, mouse embryo viability, and the mRNA levels of apoptosis related genes and miRNA-21 during mouse embryo development in vitro. Addition of 10 or 100 ng/ml of recombinant IL-6 to the culture medium did not affect the developmental ability of 2-cell stage embryos into blastocysts. However, total cell number was significantly increased and apoptosis was reduced in blastocyst stage embryos cultured in the presence of 100 ng/ml of recombinant IL-6. Furthermore, addition of recombinant IL-6 to the culture medium significantly increased the copy numbers of anti-apoptotic miRNA-21, up-regulated Bcl2l1, and down-regulated casp3. Similarly, the injection of mature miRNA-21 into cells up-regulated Bcl2l1 and down-regulated casp3. These results suggest that the induction of the Stat3 anti-apoptotic pathway by IL-6 is linked to miRNA-21 expression, which possibly results in the regulation of cell apoptosis in early mouse embryo development.
- Subjects :
- Animals
Apoptosis physiology
Apoptosis Regulatory Proteins genetics
Blastocyst cytology
Blastocyst drug effects
Cell Membrane metabolism
Cell Nucleus metabolism
Data Interpretation, Statistical
Female
Gene Expression drug effects
Interleukin-6 genetics
Interleukin-6 pharmacology
Mice
MicroRNAs analysis
Microscopy, Confocal
Microscopy, Fluorescence
Phosphorylation drug effects
RNA, Messenger genetics
RNA, Messenger metabolism
Recombinant Proteins genetics
Recombinant Proteins metabolism
Recombinant Proteins pharmacology
STAT3 Transcription Factor genetics
Signal Transduction
Apoptosis Regulatory Proteins metabolism
Blastocyst metabolism
Interleukin-6 metabolism
MicroRNAs metabolism
STAT3 Transcription Factor metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1098-2795
- Volume :
- 76
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Molecular reproduction and development
- Publication Type :
- Academic Journal
- Accession number :
- 19437447
- Full Text :
- https://doi.org/10.1002/mrd.21048