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Genistein synergizes with RNA interference inhibiting survivin for inducing DU-145 of prostate cancer cells to apoptosis.
- Source :
-
Cancer letters [Cancer Lett] 2009 Nov 01; Vol. 284 (2), pp. 189-97. Date of Electronic Publication: 2009 May 10. - Publication Year :
- 2009
-
Abstract
- To further investigate the effect of a combination of genistein with survivin of RNA interference on the proliferation and apoptosis of DU-145 cells, the effect of genistein on the proliferation of DU-145 cells was detected by the MTT method and cytometry, and the apoptosis of cells was observed with fluorescence microscopy. In order to test combined genistein with transfection of small interfering RNA (siRNA) against survivin, a survivin siRNA plasmid was constructed and transfected into DU-145 cells. Genistein inhibited proliferation and induced apoptosis of cancerous DU-145 and Hela cells, whereas genistein had minimal effects for normal L-O2 cells. The stable transfected cell lines of DU-145, knockdown survivin by siRNA, displayed stronger apoptotic than untransfected DU-145, the transfected cell of DU-145 treated with genistein demonstrated the inhibition of proliferation and induction of apoptosis significantly; it showed genistein synergistic effect with RNAi in survivin for inhibition of prostate cancer cells.<br /> (2009 Published by Elsevier Ireland Ltd.)
- Subjects :
- Caspase 3 metabolism
Cell Division drug effects
Cell Line, Tumor cytology
Cell Line, Tumor drug effects
Cells, Cultured drug effects
Drug Screening Assays, Antitumor
HeLa Cells drug effects
Hepatocytes drug effects
Humans
Inhibitor of Apoptosis Proteins
Male
Microtubule-Associated Proteins genetics
Microtubule-Associated Proteins physiology
Neoplasm Proteins genetics
Neoplasm Proteins physiology
RNA, Messenger biosynthesis
RNA, Neoplasm biosynthesis
Survivin
Transfection
Adenocarcinoma pathology
Antineoplastic Agents, Phytogenic pharmacology
Apoptosis drug effects
Genetic Vectors pharmacology
Genistein pharmacology
Microtubule-Associated Proteins antagonists & inhibitors
Neoplasm Proteins antagonists & inhibitors
Prostatic Neoplasms pathology
RNA Interference
RNA, Small Interfering pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7980
- Volume :
- 284
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Cancer letters
- Publication Type :
- Academic Journal
- Accession number :
- 19433345
- Full Text :
- https://doi.org/10.1016/j.canlet.2009.04.024