High-level biosynthesis of the anteiso-C(17) isoform of the antibiotic mycosubtilin in Bacillus subtilis and characterization of its candidacidal activity.

Authors :: Fickers P
Guez JS
Damblon C
Leclère V
Béchet M
Jacques P
Joris B
Source :: Applied and environmental microbiology [Appl Environ Microbiol] 2009 Jul; Vol. 75 (13), pp. 4636-40. Date of Electronic Publication: 2009 May 08.
Publication Year :: 2009
Abstract: High-level production (880 mg liter(-1)) and isolation of the anteiso-C(17) isoform of the lipopeptide mycosubtilin produced by a genetically engineered Bacillus subtilis strain are reported. Antifungal activity of this isoform, as determined via culture and fluorometric and cell leakage assays, suggests its potential therapeutic use as an antifungal agent, in particular against Candida spp.

Subjects :: Bacillus subtilis genetics
Biosynthetic Pathways genetics
Genes, Bacterial
Genetic Engineering
Lipoproteins biosynthesis
Microbial Sensitivity Tests
Protein Isoforms metabolism
Antifungal Agents biosynthesis
Bacillus subtilis metabolism
Candida drug effects

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