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Elevated expression of CTRP3/cartducin contributes to promotion of osteosarcoma cell proliferation.
- Source :
-
Oncology reports [Oncol Rep] 2009 Jun; Vol. 21 (6), pp. 1477-81. - Publication Year :
- 2009
-
Abstract
- CTRP3/cartducin, a novel secretory protein, is a member of the C1q and tumor necrosis factor (TNF)-related protein (CTRP) superfamily, and plays important roles in regulating both embryonic cartilage development and postnatal longitudinal bone growth. CTRP3/cartducin is expressed in human osteosarcomas. We hypothesized that CTRP3/cartducin might have a role in osteosarcoma tumor growth and metastasis. Murine osteosarcoma cell lines, NHOS and LM8, were used as a model. RT-PCR analysis showed that the mRNA level of CTRP3/cartducin was increased in these two murine osteosarcoma cell lines compared with its level in normal murine osteoblast MC3T3-E1 cells. Western blot analysis showed that the protein level of CTRP3/cartducin was also increased in these two osteosarcoma cell lines. Stimulation of NHOS and LM8 cells by CTRP3/cartducin promoted tumor cell growth but not migration in vitro. Further, CTRP3/cartducin stimulation led to the activation of extracellular signal-regulated kinase 1/2 (ERK1/2) in these two osteosarcoma cell lines. MAPK/ERK kinase 1/2 (MEK1/2) inhibitor, U0126, blocked CTRP3/cartducin-induced cell proliferation. These results suggest that CTRP3/cartducin expression may play a role in osteosarcoma tumor growth associated with activation of the ERK1/2 signaling pathway.
- Subjects :
- Adipokines
Animals
Bone Neoplasms genetics
Bone Neoplasms pathology
Cell Line, Tumor
Cell Movement
Gene Expression Regulation, Neoplastic
Mice
Mitogen-Activated Protein Kinase 1 antagonists & inhibitors
Mitogen-Activated Protein Kinase 1 metabolism
Mitogen-Activated Protein Kinase 3 antagonists & inhibitors
Mitogen-Activated Protein Kinase 3 metabolism
Osteosarcoma genetics
Osteosarcoma pathology
Phosphorylation
Protein Kinase Inhibitors pharmacology
Proteins genetics
RNA, Messenger metabolism
Recombinant Proteins metabolism
Signal Transduction
Time Factors
Up-Regulation
Bone Neoplasms metabolism
Cell Proliferation drug effects
Osteosarcoma metabolism
Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1021-335X
- Volume :
- 21
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Oncology reports
- Publication Type :
- Academic Journal
- Accession number :
- 19424626
- Full Text :
- https://doi.org/10.3892/or_00000377