Back to Search
Start Over
Changes in lipid metabolism associated gene transcripts during porcine adipogenesis.
- Source :
-
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology [Comp Biochem Physiol B Biochem Mol Biol] 2009 May; Vol. 153 (1), pp. 8-17. Date of Electronic Publication: 2009 Jan 19. - Publication Year :
- 2009
-
Abstract
- Pigs are recognised as suitable biomedical models to study obesity and obesity-related diseases; however, little is known about adipose tissue development and adipogenesis in pigs. In this study, the temporal expression of key genes involved in lipid metabolism was investigated during porcine adipogenesis and the metabolic fate of exogenously administered palmitic acid (16:0) was examined in differentiating preadipocytes. The expression of genes encoding elongases and desaturases increased simultaneously with those involved in fatty acid and triacylglycerol synthesis during porcine adipogenesis, and a high biosynthesis of monounsaturated fatty acids was measured prior to storage in differentiating preadipocytes. Although the total fatty acid oxidation in differentiating preadipocytes was low, differentiating cells showed increased expression of hormone-sensitive lipase and mitochondrial and peroxisomal genes. These data provide new insight into the temporal expression of genes involved in lipid metabolism during porcine adipogenesis and suggest a possible role of elongation and desaturation events prior to lipid accumulation in porcine adipocytes.
- Subjects :
- Acetyltransferases genetics
Adipocytes cytology
Adipose Tissue cytology
Animals
Cell Differentiation drug effects
Cell Differentiation genetics
Cells, Cultured
Cluster Analysis
Fatty Acid Desaturases genetics
Fatty Acid Elongases
Palmitic Acid metabolism
Palmitic Acid pharmacology
Reverse Transcriptase Polymerase Chain Reaction
Swine
Adipocytes metabolism
Adipogenesis genetics
Gene Expression Profiling
Lipid Metabolism genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1107
- Volume :
- 153
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 19416703
- Full Text :
- https://doi.org/10.1016/j.cbpb.2008.12.019