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Laser microdissection of neonatal porcine endometrium for tissue-specific evaluation of relaxin receptor (RXFP1) expression in response to perinatal zearalenone exposure.
- Source :
-
Annals of the New York Academy of Sciences [Ann N Y Acad Sci] 2009 Apr; Vol. 1160, pp. 190-1. - Publication Year :
- 2009
-
Abstract
- Porcine neonatal uterine relaxin receptor (RXFP1) expression is tissue compartment specific and estrogen sensitive. Here, procedures were established for laser microdissection, tissue capture, and quantification of the effects of perinatal exposure (14 days pre- to 21 days postnatal) to a selective estrogen receptor modulator of environmental origin, zearalenone (ZEA), on endometrial RXFP1 expression. Total RNA from captured endometrium was used to generate cDNA for quantitative reverse transcription-PCR. Cycle threshold values indicated that ZEA reduced (P < 0.06) endometrial RXFP1 expression on postnatal days 20-21.
- Subjects :
- Animals
Animals, Newborn
Endometrium drug effects
Female
Pregnancy
Receptors, G-Protein-Coupled genetics
Reverse Transcriptase Polymerase Chain Reaction
Swine
Endometrium metabolism
Estrogens, Non-Steroidal pharmacology
Gene Expression Regulation drug effects
Lasers
Microdissection methods
Receptors, G-Protein-Coupled metabolism
Zearalenone pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1749-6632
- Volume :
- 1160
- Database :
- MEDLINE
- Journal :
- Annals of the New York Academy of Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 19416185
- Full Text :
- https://doi.org/10.1111/j.1749-6632.2008.03821.x