Real-time high resolution melting PCR for identification of the Swedish variant of Chlamydia trachomatis.

Authors :: Raherison S
Clerc M
Trombert S
Cado S
Normandin F
Bébéar C
de Barbeyrac B
Source :: Journal of microbiological methods [J Microbiol Methods] 2009 Jul; Vol. 78 (1), pp. 101-3. Date of Electronic Publication: 2009 May 03.
Publication Year :: 2009
Abstract: We developed a real-time High Resolution Melting PCR to identify the new Swedish variant of Chlamydia trachomatis ncCT. Of 1191 urogenital specimens C. trachomatis-positive by an omp1 real-time PCR, collected in France in 2007-2008, 1128 gave an interpretable profile corresponding to the wild-type strain; no nvCT was found. This test can be used on selected C. trachomatis-positive samples to monitor the nvCT spread.

Subjects :: Chlamydia trachomatis genetics
Humans
Sensitivity and Specificity
Sweden
Transition Temperature
Chlamydia Infections microbiology
Chlamydia trachomatis isolation & purification
Genetic Variation
Polymerase Chain Reaction methods

Full Text Access

Tools