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HBx gene inducing hepatocellular carcinoma in vivo and its mechanism.
- Source :
-
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences [Zhong Nan Da Xue Xue Bao Yi Xue Ban] 2009 Apr; Vol. 34 (4), pp. 282-8. - Publication Year :
- 2009
-
Abstract
- Objective: To determine whether HBx gene can directly induce hepatocellular carcinoma in vivo, and to explore the mechanism of transplantation tumor in nude mice.<br />Methods: pCMVX/QSG7701 cell lines were vaccinated into subcutaneous tissue of nude mice. pRcCMV2/QSG7701 and QSG7701 cell lines were used as controls. The sections of transplantation tumor were observed microscopically by HE staining. RT-PCR was used to detect the expression of mutant p53 and c-Myc mRNA in transplant tumor and an other 3 cell lines.<br />Results: The transplant tumor occurred within the subcutaneous tissue of the nude mice inoculated with pCMVX/QSG7701 cell lines at 2nd week after the vaccination. No metastatic tumor was found in other organs. Transplant tumor was not formed in all the controls. HE staining confirmed that the transplant tumor was hepatocellular carcinoma. The mutant p53 mRNA and c-Myc mRNA expression level of transplant tumor and pCMVX/QSG7701 cells was significantly higher than that of pRcCMV2/QSG7701 and QSG7701 cells, respectively (P<0.01).<br />Conclusion: HBx gene can up-regulate the expression of mutant p53 and c-Myc genes, and directly induce hepatocellular carcinoma in vivo.
- Subjects :
- Animals
Cell Line, Tumor
Gene Expression Regulation, Neoplastic
Mice
Mice, Nude
Mutation
Proto-Oncogene Proteins c-myc metabolism
RNA, Messenger metabolism
Tumor Suppressor Protein p53 metabolism
Viral Regulatory and Accessory Proteins
Carcinoma, Hepatocellular pathology
Hepatitis B virus genetics
Liver Neoplasms pathology
Trans-Activators genetics
Transfection
Subjects
Details
- Language :
- English
- ISSN :
- 1672-7347
- Volume :
- 34
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
- Publication Type :
- Academic Journal
- Accession number :
- 19411742