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Mucosal immunization using proteoliposome and cochleate structures from Neisseria meningitidis serogroup B induce mucosal and systemic responses.
- Source :
-
Methods (San Diego, Calif.) [Methods] 2009 Dec; Vol. 49 (4), pp. 301-8. Date of Electronic Publication: 2009 May 04. - Publication Year :
- 2009
-
Abstract
- Most pathogens either invade the body or establish infection in mucosal tissues and represent an enormous challenge for vaccine development by the absence of good mucosal adjuvants. A proteoliposome-derived adjuvant from Neisseria meningitidis serogroup B (AFPL1, Adjuvant Finlay Proteoliposome 1) and its derived cochleate form (Co, AFCo1) contain multiple pathogen-associated molecular patterns as immunopotentiators, and can also serve as delivery systems to elicit a Th1-type immune response. The present studies demonstrate the ability of AFPL1and AFCo1 to induce mucosal and systemic immune responses by different mucosal immunizations routes and significant adjuvant activity for antibody responses of both structures: a microparticle and a nanoparticle with a heterologous antigen. Therefore, we used female mice immunized by intragastric, intravaginal, intranasal or intramuscular routes with both structures alone or incorporated with ovalbumin (OVA). High levels of specific IgG antibody were detected in all sera and in vaginal washes, but specific IgA antibody in external secretions was only detected in mucosally immunized mice. Furthermore, antigen specific IgG1 and IgG2a isotypes were all induced. AFPL1 and AFCo1 are capable of inducing IFN-gamma responses, and chemokine secretions, like MIP-1alpha and MIP-1beta. However, AFCo1 is a better alternative to induce immune responses at mucosal level. Even when we use a heterologous antigen, the AFCo1 response was better than with AFPL1 in inducing mucosal and systemic immune responses. These results support the use of AFCo1 as a potent Th1 inducing adjuvant particularly suitable for mucosal immunization.
- Subjects :
- Administration, Intranasal
Administration, Intravaginal
Animals
Cells, Cultured
Deoxycholic Acid administration & dosage
Deoxycholic Acid immunology
Edetic Acid administration & dosage
Edetic Acid immunology
Female
Immunity, Mucosal drug effects
Immunity, Mucosal immunology
Immunoglobulin A biosynthesis
Immunoglobulin G biosynthesis
Mice
Mice, Inbred BALB C
Mucous Membrane drug effects
Immunization methods
Mucous Membrane immunology
Neisseria meningitidis, Serogroup B immunology
Proteolipids administration & dosage
Proteolipids immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9130
- Volume :
- 49
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Methods (San Diego, Calif.)
- Publication Type :
- Academic Journal
- Accession number :
- 19410000
- Full Text :
- https://doi.org/10.1016/j.ymeth.2009.03.025