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[Study on the replicative and premature senescence induced by hydrogen peroxide of human embryonic lung fibroblasts].
- Source :
-
Wei sheng yan jiu = Journal of hygiene research [Wei Sheng Yan Jiu] 2009 Mar; Vol. 38 (2), pp. 139-43. - Publication Year :
- 2009
-
Abstract
- Objective: To study both cellular replicative and prematuce senescence induced by hydrogen peroxide (H2O2) of human embryonic lung fibroblasts (HEFs).<br />Methods: HEFs were cultured conventionally and treated with H2O2. Biomarkers of senescence were assessed, including cell morphology, growth curve, life span, distribution of cell cycle and senescence associated beta-galactosidase (SA-beta-gal), and the expression of proliferating cell nuclear antigen (PCNA).<br />Results: HEFs entered replicative senescence status at 52 population doubling levels (PDL) and 400 micromol/L H2O2 induced premature senescence of HEFs in a short period. Senescent cells were characterized as enlarged, flattened and reduced saturation density, retarded in the G1 phase with increased expression of SA-beta-gal. The mRNA and protein level of PCNA decreased gradually during cellular senescence.<br />Conclusion: Cellular premature senescence could be induced by 400 micromol/L H2O2. Premature senescence induced by H2O2 could share the morphology characteristics and proliferative capacity similar to those of replicative one.
- Subjects :
- Cell Proliferation drug effects
Cells, Cultured
Embryo, Mammalian
Humans
Proliferating Cell Nuclear Antigen genetics
Proliferating Cell Nuclear Antigen metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
beta-Galactosidase metabolism
Cellular Senescence drug effects
Fibroblasts cytology
Hydrogen Peroxide pharmacology
Lung cytology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1000-8020
- Volume :
- 38
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Wei sheng yan jiu = Journal of hygiene research
- Publication Type :
- Academic Journal
- Accession number :
- 19408652