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Exposure of mcf-7 breast cancer cells to electromagnetic fields up-regulates the plasminogen activator system.
- Source :
-
International journal of gynecological cancer : official journal of the International Gynecological Cancer Society [Int J Gynecol Cancer] 2009 Apr; Vol. 19 (3), pp. 334-8. - Publication Year :
- 2009
-
Abstract
- Effects of electromagnetic fields (EMFs) on the incidence of breast cancer (BC) have been proposed by a number of epidemiological studies. The molecular mechanism of the impact of EMFs on cells is not yet clear, although changes in gene expression have been reported in various cellular systems. In this investigation, the interference of low-frequency EMFs with the plasminogen activator system was examined in BC cells.MCF-7 BC cells from 2 different sources were exposed to highly homogeneous 50-Hz EMFs. Changes in gene expression were analyzed by reverse transcriptase-polymerase chain reaction.In MCF-7 cells exposed to 1.2 microT EMF expression of the urokinase plasminogen activator gene and of plasminogen-activator inhibitor-1 was markedly increased. The expression of the receptor for urokinase plasminogen activator was only marginally increased in 1 of the 2 tested cell lines and expression of the tissue plasminogen activator was at least slightly down-regulated in BC cells exposed to EMFs.EMFs may be able to increase the metastatic potential of breast tumors. The use of our newly established exposure system for EMFs may allow us to study the signaling processes involved in the induction of a metastatic phenotype of breast cancer cells.
- Subjects :
- Breast Neoplasms genetics
Breast Neoplasms pathology
Female
Humans
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured metabolism
Tumor Cells, Cultured radiation effects
Electromagnetic Fields
Plasminogen Activator Inhibitor 1 genetics
Plasminogen Activators genetics
Receptors, Urokinase Plasminogen Activator genetics
Up-Regulation genetics
Urokinase-Type Plasminogen Activator genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1525-1438
- Volume :
- 19
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- International journal of gynecological cancer : official journal of the International Gynecological Cancer Society
- Publication Type :
- Academic Journal
- Accession number :
- 19407555
- Full Text :
- https://doi.org/10.1111/IGC.0b013e31819f53ec