Purification, crystallization and preliminary X-ray diffraction analysis of a plant subtilase.

Authors :: Rose R
Huttenlocher F
Cedzich A
Kaiser M
Schaller A
Ottmann C
Source :: Acta crystallographica. Section F, Structural biology and crystallization communications [Acta Crystallogr Sect F Struct Biol Cryst Commun] 2009 May 01; Vol. 65 (Pt 5), pp. 522-5. Date of Electronic Publication: 2009 Apr 28.
Publication Year :: 2009
Abstract: The subtilase SBT3 from Solanum lycopersicum (tomato) was purified from a tomato cell culture and crystallized using the sitting-drop vapour-diffusion method. A native data set was collected to 2.5 A resolution at 100 K using synchrotron radiation. For experimental phasing, CsCl-derivative and tetrakis(acetoxymercuri)methane (TAMM) derivative crystals were employed for MIRAS phasing. Three caesium sites and one TAMM site were identified, which allowed solution of the structure.

Subjects :: Crystallization
Crystallography, X-Ray
Solanum lycopersicum genetics
Subtilisins isolation & purification
Subtilisins metabolism
Solanum lycopersicum enzymology
Subtilisins analysis
Subtilisins chemistry

Language :: English
ISSN :: 1744-3091
Volume :: 65
Issue :: Pt 5
Database :: MEDLINE
Journal :: Acta crystallographica. Section F, Structural biology and crystallization communications
Publication Type :: Academic Journal
Accession number :: 19407393
Full Text :: https://doi.org/10.1107/S1744309109013979

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