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Cloning, purification, crystallization and preliminary X-ray analysis of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana.
- Source :
-
Acta crystallographica. Section F, Structural biology and crystallization communications [Acta Crystallogr Sect F Struct Biol Cryst Commun] 2009 May 01; Vol. 65 (Pt 5), pp. 478-81. Date of Electronic Publication: 2009 Apr 24. - Publication Year :
- 2009
-
Abstract
- The receiver domain (RD) of a sensor histidine kinase (HK) catalyses the transphosphorylation reaction during the action of HKs in hormonal and abiotic signalling in plants. Crystals of the recombinant RD of the Arabidopsis thaliana HK CYTOKININ-INDEPENDENT1 (CKI1(RD)) have been obtained by the hanging-drop vapour-diffusion method using ammonium sulfate as a precipitant and glycerol as a cryoprotectant. The crystals diffracted to approximately 2.4 A resolution on beamline BW7B of the DORIS-III storage ring. The diffraction improved significantly after the use of a non-aqueous cryoprotectant. Crystals soaked in Paratone-N diffracted to at least 2.0 A resolution on beamline BW7B and their mosaicity decreased more than tenfold. The crystals belonged to space group C222(1), with unit-cell parameters a = 54.46, b = 99.82, c = 79.94 A. Assuming the presence of one molecule of the protein in the asymmetric unit gives a Matthews coefficient V(M) of 2.33 A(3) Da(-1). A molecular-replacement solution has been obtained and structure refinement is in progress.
- Subjects :
- Arabidopsis genetics
Arabidopsis Proteins isolation & purification
Arabidopsis Proteins metabolism
Cloning, Molecular
Crystallization
Crystallography, X-Ray
Histidine Kinase
Protein Kinases isolation & purification
Protein Kinases metabolism
Arabidopsis enzymology
Arabidopsis Proteins chemistry
Protein Kinases chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1744-3091
- Volume :
- 65
- Issue :
- Pt 5
- Database :
- MEDLINE
- Journal :
- Acta crystallographica. Section F, Structural biology and crystallization communications
- Publication Type :
- Academic Journal
- Accession number :
- 19407381
- Full Text :
- https://doi.org/10.1107/S1744309109012032