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Comparison of semen profile and frequency of chromosome aneuploidies in sperm nuclei of patients with varicocele before and after varicocelectomy.
- Source :
-
Andrologia [Andrologia] 2009 Jun; Vol. 41 (3), pp. 157-62. - Publication Year :
- 2009
-
Abstract
- Semen profile and meiotic segregation products are important for assessing aneuploidy risk and risk of resulting infertility. To determine the effect of varicocelectomy on semen profile and aneuploidy frequency, we investigated semen profile and aneuploidy frequency of selected chromosomes in patients with varicocele before and after varicocelectomy. Chromosomal aneuploidy for selected chromosomes was evaluated using chromosome-specific DNA fluorescence in situ hybridisation (FISH) probes. There was a significant difference in the level of normal sperm morphology before and after varicocelectomy (P > 0.007). There were no significant differences in aneuploidy frequency of chromosomes 1, 16, 17 and 18 in sperm nuclei obtained from patients before varicocelectomy compared with 6-7 months after varicocelectomy (P > 0.05), although FISH analysis with chromosomes 17 and 18 combination showed a higher aneuploidy frequency before varicocelectomy than after varicocelectomy (7.81 +/- 9.67 versus 4.03 +/- 1.46 respectively). In conclusion, varicocele seems to affect the semen profile but minimally affects aneuploidy frequency. Varicocelectomy demonstrates a repairing effect on the semen profile and contributes to a slight decrease in aneuploidy frequency in some but not all chromosomes.
- Subjects :
- Adult
Cell Nucleus genetics
Cell Nucleus ultrastructure
Chromosomes, Human, Pair 1 ultrastructure
Chromosomes, Human, Pair 16 ultrastructure
Chromosomes, Human, Pair 17 ultrastructure
Chromosomes, Human, Pair 18 ultrastructure
Humans
In Situ Hybridization, Fluorescence
Infertility, Male etiology
Male
Varicocele complications
Varicocele surgery
Aneuploidy
Semen Analysis
Varicocele genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1439-0272
- Volume :
- 41
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Andrologia
- Publication Type :
- Academic Journal
- Accession number :
- 19400849
- Full Text :
- https://doi.org/10.1111/j.1439-0272.2008.00907.x