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[A procedure for determination of acid mannosidase activities in biological fluids].

Authors :
Sharaev PN
Zamiatin AB
Kil'diiarova RR
Parulava NSh
Khobta IuV
Source :
Klinicheskaia laboratornaia diagnostika [Klin Lab Diagn] 2009 Mar (3), pp. 9-10.
Publication Year :
2009

Abstract

A procedure has been developed for simultaneous determination of the activities of alpha-D- and beta-D-mannosidase in the biological fluids from the quantity of free 4-nitrophenol. The latter is released via enzymatic degradation of substrates of 4-nitrophenyl-alpha-D-mannose and 4-nitrophenyl-beta-D-mannose in individual incubation tests.

Details

Language :
Russian
ISSN :
0869-2084
Issue :
3
Database :
MEDLINE
Journal :
Klinicheskaia laboratornaia diagnostika
Publication Type :
Academic Journal
Accession number :
19391238