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Morphometric analysis of testis cord formation in Sox9-EGFP mice.
- Source :
-
Developmental dynamics : an official publication of the American Association of Anatomists [Dev Dyn] 2009 May; Vol. 238 (5), pp. 1100-10. - Publication Year :
- 2009
-
Abstract
- Sox9-EGFP knockin mice were generated to label Sertoli cells and visualize testis cord formation during development. Confocal microscopy and morphometric analysis of developing cords were performed. Serial histological sections were used for three-dimensional cord reconstruction. Initially, gonad length decreased from embryonic day (E) 11.5 to E13.5, but increased thereafter, while gonad width doubled every 12 hours from E11.5 through E14.5. At E12.5, the average number of cords was 12.5, whereas this decreased to 10.4 at E13.5 and E14.5. Cord number at a given time point varied between gonads and influenced dimensions. The initial cords that formed were complex and branches were common. Time-lapse imaging revealed an intricate behavior of the Sertoli-germ cell mass and cellular exchange between connected neighboring cords. These results suggest that cord formation is a highly dynamic process that subsequently becomes refined to establish the final number of seminiferous tubule precursors.
- Subjects :
- Animals
Gene Knock-In Techniques
Green Fluorescent Proteins genetics
Green Fluorescent Proteins metabolism
Male
Mice
Mice, Mutant Strains
SOX9 Transcription Factor genetics
Sertoli Cells cytology
Sertoli Cells physiology
Testis metabolism
SOX9 Transcription Factor metabolism
Testis anatomy & histology
Testis embryology
Subjects
Details
- Language :
- English
- ISSN :
- 1058-8388
- Volume :
- 238
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Developmental dynamics : an official publication of the American Association of Anatomists
- Publication Type :
- Academic Journal
- Accession number :
- 19384968
- Full Text :
- https://doi.org/10.1002/dvdy.21954