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The DEAD box protein Ddx42p modulates the function of ASPP2, a stimulator of apoptosis.
- Source :
-
Oncogene [Oncogene] 2009 May 21; Vol. 28 (20), pp. 2065-73. Date of Electronic Publication: 2009 Apr 20. - Publication Year :
- 2009
-
Abstract
- Ddx42p is a recently characterized mammalian DEAD box protein with unknown cellular function. We found that in human cells Ddx42p physically interacts with ASPP2, a major apoptosis inducer known to enhance p53 transactivation of proapoptotic genes. The proteins interact via a domain within the carboxy-terminal part of Ddx42p and a mid-amino-terminal sequence as well as the ankyrin-SH3 region of ASPP2. Overexpression of Ddx42p interferes with apoptosis induction by ASPP2, whereas Ddx42p knockdown reduces the survival rate of cultured human cells. In addition, ASPP2 is found in cytoplasm and nucleus at low Ddx42p level, and predominantly in cytoplasm at high concentration of Ddx42p, respectively. Our results show that Ddx42p is capable of modulating ASPP2 function.
- Subjects :
- Ankyrins metabolism
Apoptosis Regulatory Proteins
Carrier Proteins genetics
Cell Nucleus metabolism
Cytoplasm metabolism
DEAD-box RNA Helicases antagonists & inhibitors
DEAD-box RNA Helicases genetics
Fluorescent Antibody Technique
Gene Library
HeLa Cells
Humans
Immunoprecipitation
Protein Transport
RNA, Small Interfering pharmacology
Ribonucleoprotein, U2 Small Nuclear antagonists & inhibitors
Ribonucleoprotein, U2 Small Nuclear genetics
Transcriptional Activation
Tumor Suppressor Protein p53 genetics
Tumor Suppressor Protein p53 metabolism
Two-Hybrid System Techniques
src Homology Domains
Apoptosis physiology
Carrier Proteins metabolism
DEAD-box RNA Helicases metabolism
Ribonucleoprotein, U2 Small Nuclear metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1476-5594
- Volume :
- 28
- Issue :
- 20
- Database :
- MEDLINE
- Journal :
- Oncogene
- Publication Type :
- Academic Journal
- Accession number :
- 19377511
- Full Text :
- https://doi.org/10.1038/onc.2009.75