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Establishment and validation of a standard protocol for the detection of minimal residual disease in B lineage childhood acute lymphoblastic leukemia by flow cytometry in a multi-center setting.
- Source :
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Haematologica [Haematologica] 2009 Jun; Vol. 94 (6), pp. 870-4. Date of Electronic Publication: 2009 Apr 18. - Publication Year :
- 2009
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Abstract
- Minimal residual disease detection, used for clinical management of children with acute lymphoblastic leukemia, can be performed by molecular analysis of antigen-receptor gene rearrangements or by flow cytometric analysis of aberrant immunophenotypes. For flow minimal residual disease to be incorporated into larger national and international trials, a quality assured, standardized method is needed which can be performed in a multi-center setting. We report a four color, flow cytometric protocol established and validated by the UK acute lymphoblastic leukemia Flow minimal residual disease group. Quality assurance testing gave high inter-laboratory agreement with no values differing from a median consensus value by more than one point on a logarithmic scale. Prospective screening of B-ALL patients (n=206) showed the method was applicable to 88.3% of patients. The minimal residual disease in bone marrow aspirates was quantified and compared to molecular data. The combined risk category concordance (minimal residual disease levels above or below 0.01%) was 86% (n=134). Thus, this standardized protocol is highly reproducible between laboratories, sensitive, applicable, and shows good concordance with molecular-based analysis.
- Subjects :
- Antigens, CD19 analysis
Antigens, CD34 analysis
Child
Flow Cytometry standards
Gene Rearrangement
Humans
Leukemia, B-Cell genetics
Leukemia, B-Cell metabolism
Neoplasm, Residual genetics
Neoplasm, Residual metabolism
Neprilysin analysis
Polymerase Chain Reaction
Precursor Cell Lymphoblastic Leukemia-Lymphoma genetics
Precursor Cell Lymphoblastic Leukemia-Lymphoma metabolism
Prognosis
Prospective Studies
Receptors, Antigen, T-Cell genetics
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Flow Cytometry methods
Leukemia, B-Cell diagnosis
Neoplasm, Residual diagnosis
Precursor Cell Lymphoblastic Leukemia-Lymphoma diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1592-8721
- Volume :
- 94
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Haematologica
- Publication Type :
- Academic Journal
- Accession number :
- 19377076
- Full Text :
- https://doi.org/10.3324/haematol.2008.000414