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Discovering protein kinase C active plants growing in Finland utilizing automated bioassay combined to LC/MS.

Authors :
Galkin A
Jokela J
Wahlsten M
Tammela P
Sivonen K
Vuorela P
Source :
Natural product communications [Nat Prod Commun] 2009 Jan; Vol. 4 (1), pp. 139-42.
Publication Year :
2009

Abstract

Protein kinase C (PKC) modulating activity of 81 plant extracts of Finnish origin was investigated with an automated bioassay method combined to LC/MS. Twenty-one extracts from different parts of the plants inhibited PKC significantly. Fractionation of the active extract of Filipendula ulmaria showed that this method was able to identify a PKC inhibiting compound from the extract as quercetin. Our results indicate that this method is suitable for PKC screening of complex matrices and provides a quick and low volume, non-radioactive, alternative method for PKC experiments.

Details

Language :
English
ISSN :
1934-578X
Volume :
4
Issue :
1
Database :
MEDLINE
Journal :
Natural product communications
Publication Type :
Academic Journal
Accession number :
19370892