Back to Search
Start Over
Three-dimensional bioprinting of rat embryonic neural cells.
- Source :
-
Neuroreport [Neuroreport] 2009 May 27; Vol. 20 (8), pp. 798-803. - Publication Year :
- 2009
-
Abstract
- We present a direct cell printing technique to pattern neural cells in a three-dimensional (3D) multilayered collagen gel. A layer of collagen precursor was printed to provide a scaffold for the cells, and the rat embryonic neurons and astrocytes were subsequently printed on the layer. A solution of sodium bicarbonate was applied to the cell containing collagen layer as nebulized aerosols, which allowed the gelation of the collagen. This process was repeated layer-by-layer to construct the 3D cell-hydrogel composites. Upon characterizing the relationship between printing resolutions and the growth of printed neural cells, single/multiple layers of neural cell-hydrogel composites were constructed and cultured. The on-demand capability to print neural cells in a multilayered hydrogel scaffold offers flexibility in generating artificial 3D neural tissue composites.
- Subjects :
- Animals
Artificial Organs
Biocompatible Materials
Cell Aggregation drug effects
Cell Aggregation physiology
Cell Culture Techniques
Cell Proliferation drug effects
Cell Survival physiology
Cells, Cultured
Collagen pharmacology
Culture Media chemistry
Culture Media pharmacology
Extracellular Matrix chemistry
Hydrogels pharmacology
Neurons cytology
Neurons drug effects
Organ Culture Techniques
Rats
Spheroids, Cellular cytology
Spheroids, Cellular physiology
Stem Cells cytology
Stem Cells drug effects
Neurons physiology
Printing methods
Stem Cells physiology
Tissue Engineering methods
Tissue Scaffolds trends
Subjects
Details
- Language :
- English
- ISSN :
- 1473-558X
- Volume :
- 20
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Neuroreport
- Publication Type :
- Academic Journal
- Accession number :
- 19369905
- Full Text :
- https://doi.org/10.1097/WNR.0b013e32832b8be4