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Targeted disruption of the murine homeodomain-interacting protein kinase-2 causes growth deficiency in vivo and cell cycle arrest in vitro.
- Source :
-
DNA and cell biology [DNA Cell Biol] 2009 Apr; Vol. 28 (4), pp. 161-7. - Publication Year :
- 2009
-
Abstract
- The homeodomain-interacting protein kinase 2 (HIPK2) protein is a member of a recently identified family of nuclear protein kinases that are well conserved in various organisms. HIPK2 can bind to several homeotic factors and to a series of proteins involved in the regulation of cell survival and proliferation in response to morphogenetic and genotoxic signals. Here we report Hipk2-targeted disruption in mouse; Hipk2(-/-) mice are viable and fertile but significantly smaller than their wild-type littermates. This feature is present at birth and retained throughout the mouse adulthood. Mouse embryo fibroblasts from Hipk2(-/-) mice show a reduced proliferation rate, compared to the wild-type counterparts, with accumulation in the G0/G1 phase of the cell cycle and altered levels of the cell cycle regulators cyclin D and CDK6. Restoration of wild-type HIPK2 expression in Hipk2(-/-) cells rescues the normal phenotype supporting a role for HIPK2 in the regulation of cell proliferation.
Details
- Language :
- English
- ISSN :
- 1557-7430
- Volume :
- 28
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- DNA and cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 19364276
- Full Text :
- https://doi.org/10.1089/dna.2008.0778