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Metabolite analysis in Curcuma domestica using various GC-MS and LC-MS separation and detection techniques.
- Source :
-
Biomedical chromatography : BMC [Biomed Chromatogr] 2009 Sep; Vol. 23 (9), pp. 951-65. - Publication Year :
- 2009
-
Abstract
- The metabolic profile of polar (methanol) and non-polar (hexane) extracts of Curcuma domestica, a widely used medicinal plant, was established using various different analytical techniques, including GC-FID, GC-MS, HR-GC-MS and analytical HPLC-ESI-MS/MS by means of LTQ-Orbitrap technology. The major non-volatile curcuminoids curcumin, demethoxycurcumin and bisdemethoxycurcumin were identified when their chromatographic and precursor ion masses were compared with those of authentic standard compounds. In this paper we describe for the first time a GC/MS-based method for metabolic profiling of the hydrophilic extract. We also identified 61 polar metabolites as TMS derivatives.<br /> (Copyright (c) 2009 John Wiley & Sons, Ltd.)
- Subjects :
- Curcumin analogs & derivatives
Curcumin analysis
Curcumin isolation & purification
Diarylheptanoids
Hexanes chemistry
Methanol chemistry
Plant Extracts chemistry
Chromatography, High Pressure Liquid methods
Curcuma chemistry
Curcuma metabolism
Gas Chromatography-Mass Spectrometry methods
Plant Extracts analysis
Spectrometry, Mass, Electrospray Ionization methods
Subjects
Details
- Language :
- English
- ISSN :
- 1099-0801
- Volume :
- 23
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Biomedical chromatography : BMC
- Publication Type :
- Academic Journal
- Accession number :
- 19358151
- Full Text :
- https://doi.org/10.1002/bmc.1207