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Azithromycin fails to reduce increased expression of neutrophil-related cytokines in primary-cultured epithelial cells from cystic fibrosis mice.
- Source :
-
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society [J Cyst Fibros] 2009 May; Vol. 8 (3), pp. 203-10. Date of Electronic Publication: 2009 Apr 03. - Publication Year :
- 2009
-
Abstract
- Background: Beneficial effects of azithromycin in cystic fibrosis (CF) have been reported, however, its mechanism of action remains unclear. The present study aimed at investigating the effect of azithromycin on CF airway epithelial cells.<br />Methods: Primary cultures of purified tracheal epithelial cells from F508del and normal homozygous mice were established. Responses to lipopolysaccharide from Pseudomonas aeruginosa (LPS, 0.1 microg/ml) on mRNA expression of neutrophil-related chemokines, pro- and anti-inflammatory cytokines were investigated in the presence or the absence of azithromycin (1 microg/ml).<br />Results: CF airway epithelial cells showed upregulation of MIP-2 and KC responses to LPS, and azithromycin failed to downregulate these responses. In contrast, in CF cells, azithromycin increased KC and TNF-alpha expression under non-stimulated and LPS-stimulated conditions, respectively. In non-CF cells, the macrolide potentiated the LPS response on MIP-2 and on IL-10.<br />Conclusions: Airway epithelial cells contribute to the dysregulated immune processes in CF. Azithromycin rather stimulates cytokine expression in CF airway epithelial cells.
- Subjects :
- Animals
Cell Culture Techniques
Cells, Cultured drug effects
Cells, Cultured immunology
Cystic Fibrosis immunology
Cystic Fibrosis metabolism
Disease Models, Animal
Epithelial Cells drug effects
Epithelial Cells immunology
Mice
Mice, Inbred Strains
Neutrophils drug effects
Neutrophils metabolism
RNA, Messenger drug effects
RNA, Messenger metabolism
Trachea cytology
Trachea drug effects
Trachea immunology
Anti-Bacterial Agents administration & dosage
Azithromycin administration & dosage
Cystic Fibrosis drug therapy
Cytokines drug effects
Cytokines metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-5010
- Volume :
- 8
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society
- Publication Type :
- Academic Journal
- Accession number :
- 19345617
- Full Text :
- https://doi.org/10.1016/j.jcf.2009.03.003