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High-sensitivity detection of fruit tree viruses using bacterial magnetic particles.
- Source :
-
Journal of integrative plant biology [J Integr Plant Biol] 2009 Apr; Vol. 51 (4), pp. 409-13. - Publication Year :
- 2009
-
Abstract
- Prunus necrotic ring spot virus (PNRSV) and grapevine fanleaf virus (GFLV) were detected by fluoroimmunoassay using bacterial magnetic particles (BMPs), and a double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA). For the fluoroimmunoassay, fluorescein isothiocyanate labeled anti-PNRSV antibody or anti-GFLV antibody was conjugated onto BMPs of Magnetospirillum gryphiswaldense MSR-1. With this method, a very low minimum antigen concentration (1 x 10(6) dilution of the original sample concentration) could be detected. Using DAS-ELISA, the minimum antigen detection concentration was the original sample concentration. Thus, comparing these two methods, a BMP-based method could increase the sensitivity up to six orders of magnitude (10(6)) higher than an ELISA-based method of detection PNRSV and GFLV.
Details
- Language :
- English
- ISSN :
- 1744-7909
- Volume :
- 51
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of integrative plant biology
- Publication Type :
- Academic Journal
- Accession number :
- 19341408
- Full Text :
- https://doi.org/10.1111/j.1744-7909.2008.00785.x