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The role of oxygen-free radical in the apoptosis of enterocytes and bacterial translocation in abdominal compartment syndrome.
- Source :
-
Free radical research [Free Radic Res] 2009 May; Vol. 43 (5), pp. 470-7. - Publication Year :
- 2009
-
Abstract
- Background: The purpose of this study was to study the impact of intra-abdominal hypertension (IAH) on the intestine.<br />Materials and Methods: One hundred and twenty Sprague-Daley rats were divided into four groups. In the ACS group, the intra-abdominal pressure (IAP) was increased to 20 mmHg. In the ACS/DE group, increased IAP was followed by decompression. In the control1 and control2 groups, the IAP remained unchanged. Malondialdehyde (MDA), myeloperoxidase (MPO), glutathione (GSH) and glutathione peroxidase (GSH-Px) enzymes of the intestine were measured. Additionally, ileal tissues were obtained for histopathological examinations and apoptosis detection. Liver, spleen and mesenteric lymph nodes were obtained for microbiological analysis.<br />Results: In the presence of IAH, MDA and MPO were increased, while GSH and GSH-Px were decreased. Microbiological analysis suggested bacterial translocation across the gut. Morphological examinations indicated that the Chiu's score and apoptotic index in the ACS/DE group were the highest in the four groups.<br />Conclusions: Oxidative stress plays an important role in the intestinal damage and bacterial translocation in abdominal compartment syndrome. Additionally, the influence of oxygen free radicals occurs mainly during the period of reperfusion rather than during the IAH period.
- Subjects :
- Abdomen
Animals
Compartment Syndromes pathology
Disease Models, Animal
Glutathione metabolism
Glutathione Peroxidase metabolism
Intestinal Mucosa metabolism
Intestinal Mucosa microbiology
Intestinal Mucosa pathology
Male
Malondialdehyde metabolism
Oxidative Stress
Peroxidase metabolism
Pressure
Rats
Rats, Sprague-Dawley
Apoptosis physiology
Compartment Syndromes metabolism
Compartment Syndromes microbiology
Enterocytes metabolism
Enterocytes pathology
Reactive Oxygen Species metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1029-2470
- Volume :
- 43
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Free radical research
- Publication Type :
- Academic Journal
- Accession number :
- 19340666
- Full Text :
- https://doi.org/10.1080/10715760902846157