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Recombinant human granulocyte macrophage colony-stimulating factor (rhGM-CSF) induces human macrophage production of transforming growth factor-alpha.
- Source :
-
Cellular and molecular biology [Cell Mol Biol] 1991; Vol. 37 (4), pp. 413-9. - Publication Year :
- 1991
-
Abstract
- Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is known as an inducer of proliferation and functional activation of myeloid cells. This study was carried out to characterize the effect of purified recombinant human GM-CSF (rhGM-CSF) on induction of TGF-alpha in macrophages. Using Northern blot analysis and immunoassays, we show here that rhGM-CSF markedly stimulates production of TGF-alpha messenger RNA and protein in normal tonsil macrophages. The findings are consistent with macrophages being a normal inducible source of TGF-alpha which may be an important mediator of various activities of GM-CSF both in hematopoietic and non-hematopoietic cells.
- Subjects :
- Animals
Blotting, Northern
Cells, Cultured
Colony-Forming Units Assay
Dose-Response Relationship, Drug
Enzyme-Linked Immunosorbent Assay
Humans
Kinetics
Rats
Recombinant Proteins pharmacology
Granulocyte-Macrophage Colony-Stimulating Factor pharmacology
Macrophages metabolism
Transforming Growth Factor alpha biosynthesis
Subjects
Details
- Language :
- English
- Volume :
- 37
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Cellular and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 1934015