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Validation of an integrated Raman- and angular-scattering microscopy system on heterogeneous bead mixtures and single human immune cells.
Validation of an integrated Raman- and angular-scattering microscopy system on heterogeneous bead mixtures and single human immune cells.
- Source :
-
Applied optics [Appl Opt] 2009 Apr 01; Vol. 48 (10), pp. D109-20. - Publication Year :
- 2009
-
Abstract
- A microscopy system has been constructed that is capable of simultaneously acquiring both Raman spectra and angle-resolved elastic-scattering patterns in either epi- or transillumination modes with a 7 mum spot size. The benefits and drawbacks of the epi- and transillumination modalities are discussed. Validation studies have been performed on single beads of a few micrometers in size, as well as on ensembles of submicrometer particles. In addition, transilluminated Raman and elastic-scattering spectra were obtained from single granulocytes and peripheral blood monocytes. Both the Raman- and the elastic-scattering channels show clear differences between the two types of immune cells.
Details
- Language :
- English
- ISSN :
- 1539-4522
- Volume :
- 48
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Applied optics
- Publication Type :
- Academic Journal
- Accession number :
- 19340098
- Full Text :
- https://doi.org/10.1364/ao.48.00d109