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Validation of an integrated Raman- and angular-scattering microscopy system on heterogeneous bead mixtures and single human immune cells.

Validation of an integrated Raman- and angular-scattering microscopy system on heterogeneous bead mixtures and single human immune cells.

Authors :
Smith ZJ
Berger AJ
Source :
Applied optics [Appl Opt] 2009 Apr 01; Vol. 48 (10), pp. D109-20.
Publication Year :
2009

Abstract

A microscopy system has been constructed that is capable of simultaneously acquiring both Raman spectra and angle-resolved elastic-scattering patterns in either epi- or transillumination modes with a 7 mum spot size. The benefits and drawbacks of the epi- and transillumination modalities are discussed. Validation studies have been performed on single beads of a few micrometers in size, as well as on ensembles of submicrometer particles. In addition, transilluminated Raman and elastic-scattering spectra were obtained from single granulocytes and peripheral blood monocytes. Both the Raman- and the elastic-scattering channels show clear differences between the two types of immune cells.

Details

Language :
English
ISSN :
1539-4522
Volume :
48
Issue :
10
Database :
MEDLINE
Journal :
Applied optics
Publication Type :
Academic Journal
Accession number :
19340098
Full Text :
https://doi.org/10.1364/ao.48.00d109