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Anti-inflammatory activity of (-)-aptosimon isolated from Daphne genkwa in RAW264.7 cells.
- Source :
-
International immunopharmacology [Int Immunopharmacol] 2009 Jul; Vol. 9 (7-8), pp. 878-85. Date of Electronic Publication: 2009 Mar 26. - Publication Year :
- 2009
-
Abstract
- In the present study, we investigated that (-)-aptosimon, isolated from flower buds of Daphne genkwa, inhibited cyclooxygenase-2 (COX-2) and inducible nitric oxide (NO) synthase (iNOS) expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Similarly, (-)-aptosimon suppressed tumor necrosis factor (TNF)-alpha production. Our results clearly indicated that (-)-aptosimon inhibited LPS-induced nuclear factor-kappaB (NF-kappaB) activation, by preventing degradation of the inhibitor kappa B-alpha (IkappaB-alpha). (-)-Aptosimon also inhibited interleukin-4 (IL-4) and interleukin-13 (IL-13) production in ConA-induced splenocytes. In conclusion, the anti-inflammatory effects of (-)-aptosimon are attributed to the suppression of pro-inflammatory cytokines and mediators by blocking NF-kappaB activation. These data suggest that (-)-aptosimon as a potential therapeutic agent for inflammation-associated disorders.
- Subjects :
- Animals
Cell Line
Concanavalin A metabolism
Cyclooxygenase 2 genetics
Cyclooxygenase 2 metabolism
Enzyme Activation drug effects
Enzyme Activation immunology
Flowers
Interleukin-13 genetics
Interleukin-13 metabolism
Interleukin-4 genetics
Interleukin-4 metabolism
Lipopolysaccharides metabolism
Macrophages immunology
Macrophages metabolism
Macrophages pathology
Mice
NF-kappa B antagonists & inhibitors
Nitric Oxide Synthase Type II genetics
Nitric Oxide Synthase Type II metabolism
Transcriptional Activation drug effects
Tumor Necrosis Factor-alpha genetics
Tumor Necrosis Factor-alpha metabolism
Anti-Inflammatory Agents, Non-Steroidal pharmacology
Daphne
Dioxoles pharmacology
Furans pharmacology
Macrophages drug effects
Phytotherapy trends
Subjects
Details
- Language :
- English
- ISSN :
- 1878-1705
- Volume :
- 9
- Issue :
- 7-8
- Database :
- MEDLINE
- Journal :
- International immunopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 19328870
- Full Text :
- https://doi.org/10.1016/j.intimp.2009.03.012