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One step purification of the grape vacuolar invertase.
- Source :
-
Analytica chimica acta [Anal Chim Acta] 2009 Apr 06; Vol. 638 (1), pp. 75-8. Date of Electronic Publication: 2009 Feb 11. - Publication Year :
- 2009
-
Abstract
- Invertase is a major protein of grape juice and wine. Accordingly, in order to study the biochemical and structural characteristics of this protein and for a better understanding of its physico-chemical properties, large amounts of the pure protein are needed. A simple method for the purification of the grape vacuolar invertase in a preparative-scale is described in this work. The grape protein was isolated and purified from must by ultrafiltration and anion exchange chromatography. The identification and purity determination of the grape invertase fraction were assessed by SDS-PAGE, and were then confirmed using nanoLC-chip-MS/MS analysis. The laboratory fractionation procedure presented in this work generated large quantities of pure grape vacuolar invertase from must.
Details
- Language :
- English
- ISSN :
- 1873-4324
- Volume :
- 638
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Analytica chimica acta
- Publication Type :
- Academic Journal
- Accession number :
- 19298882
- Full Text :
- https://doi.org/10.1016/j.aca.2009.02.003