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Islet beta cell failure in the 60% pancreatectomised obese hyperlipidaemic Zucker fatty rat: severe dysfunction with altered glycerolipid metabolism without steatosis or a falling beta cell mass.
- Source :
-
Diabetologia [Diabetologia] 2009 Jun; Vol. 52 (6), pp. 1122-32. Date of Electronic Publication: 2009 Mar 18. - Publication Year :
- 2009
-
Abstract
- Aims/hypothesis: The Zucker fatty (ZF) rat subjected to 60% pancreatectomy (Px) develops moderate diabetes by 3 weeks. We determined whether a progressive fall in beta cell mass and/or beta cell dysfunction contribute to beta cell failure in this type 2 diabetes model.<br />Methods: Partial (60%) or sham Px was performed in ZF and Zucker lean (ZL) rats. At 3 weeks post-surgery, beta cell mass and proliferation, proinsulin biosynthesis, pancreatic insulin content, insulin secretion, and islet glucose and lipid metabolism were measured.<br />Results: ZL-Px rats maintained normal glycaemia and glucose-stimulated insulin secretion (GSIS) despite incomplete recovery of beta cell mass possibly due to compensatory enhanced islet glucose metabolism and lipolysis. ZF-Px rats developed moderate hyperglycaemia (14 mmol/l), hypertriacylglycerolaemia and relative hypoinsulinaemia. Despite beta cell mass recovery and normal arginine-induced insulin secretion, GSIS and pancreatic insulin content were profoundly lowered in ZF-Px rats. Proinsulin biosynthesis was not reduced. Compensatory increases in islet glucose metabolism above those observed in ZF-Sham rats were not seen in ZF-Px rats. Triacylglycerol content was not increased in ZF-Px islets, possibly due to lipodetoxification by enhanced lipolysis and fatty acid oxidation. Fatty acid accumulation into monoacylglycerol and diacylglycerol was increased in ZF-Px islets together with a 4.5-fold elevation in stearoyl-CoA desaturase mRNA expression.<br />Conclusions/interpretation: Falling beta cell mass, reduced proinsulin biosynthesis and islet steatosis are not implicated in early beta cell failure and glucolipotoxicity in ZF-Px rats. Rather, severe beta cell dysfunction with a specific reduction in GSIS and marked depletion of beta cell insulin stores with altered lipid partitioning underlie beta cell failure in this animal model of type 2 diabetes.
- Subjects :
- Animals
Body Weight
Cell Proliferation
Cells, Cultured
Fatty Acids, Nonesterified metabolism
Hyperlipidemias physiopathology
Immunohistochemistry
Insulin metabolism
Insulin-Secreting Cells cytology
Insulin-Secreting Cells metabolism
Lipid Metabolism physiology
Male
Obesity physiopathology
Pancreatectomy
Proinsulin metabolism
Rats
Rats, Zucker
Hyperlipidemias metabolism
Hyperlipidemias pathology
Insulin-Secreting Cells pathology
Islets of Langerhans pathology
Obesity metabolism
Obesity pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0428
- Volume :
- 52
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Diabetologia
- Publication Type :
- Academic Journal
- Accession number :
- 19294363
- Full Text :
- https://doi.org/10.1007/s00125-009-1317-8