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11beta-hydroxysteroid dehydrogenase type 1 expression in periprosthetic osteolysis.
- Source :
-
Orthopedics [Orthopedics] 2008 Jun; Vol. 31 (6), pp. 545. - Publication Year :
- 2008
-
Abstract
- Periprosthetic membranes contain fibroblasts, macrophages and cytokines including interleukin 1Beta (IL-1Beta) and tumor necrosis factor alpha (TNF-alpha). Glucocorticoids may play an inhibitory role in osteolysis through the upregulation of 11Beta-hydroxysteroid dehydrogenase type 1 (11Beta-HSD1) in membrane fibroblasts by IL-1Beta and TNF-alpha. This study evaluated 15 periprosthetic membranes for the presence of 11Beta-HSD1 using immunochemistry. Also, fibroblast cell cultures were exposed to IL-1Beta and TNF-alpha, and 11Beta-HSD1 gene expression was evaluated. In all membranes, 11Beta-HSD1was present, and fibroblast 11Beta-HSD1 was upregulated significantly after the addition of IL-1Beta and TNF-alpha. These findings suggest increased 11Beta-HSD1 expression in fibroblasts may be a mechanism for increasing local cortisol levels in membranes, which could potentiate the osteolytic process through inhibition of osteoblastic function.
Details
- Language :
- English
- ISSN :
- 0147-7447
- Volume :
- 31
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Orthopedics
- Publication Type :
- Academic Journal
- Accession number :
- 19292355