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A mass spectrometry based imaging method developed for the intracellular detection of HIV protease inhibitors.
- Source :
-
Rapid communications in mass spectrometry : RCM [Rapid Commun Mass Spectrom] 2009 Apr; Vol. 23 (8), pp. 1183-8. - Publication Year :
- 2009
-
Abstract
- Mass spectrometry imaging is a promising technique for measuring drugs and drug metabolites in cells and tissues. In this manuscript we describe a method for the imaging of HIV protease inhibitors. As a model system we used Mono Mac 6 cells cultured with the HIV protease inhibitors saquinavir and nelfinavir deposited on glass slides using a cytocentrifuge. A sublimation/deposition device for homogeneous matrix deposition was constructed which allows imaging of these HIV protease inhibitors at clinically relevant concentrations. Using this matrix sublimation/deposition method, glass slides containing the cytocentrifuged cells can be measured and analyzed by two types of mass spectrometry techniques, viz. matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and MALDI Fourier transform ion cyclotron resonance (FTICR), and this makes it possible to perform imaging rapidly (MALDI-TOF) and with a very high selectivity (MALDI-FTICR).
- Subjects :
- Cell Line
Cyclotrons
Equipment Design
Nelfinavir analysis
Saquinavir analysis
Sensitivity and Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods
Spectroscopy, Fourier Transform Infrared methods
Time Factors
HIV Protease Inhibitors analysis
Image Enhancement methods
Mass Spectrometry methods
Subjects
Details
- Language :
- English
- ISSN :
- 0951-4198
- Volume :
- 23
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Rapid communications in mass spectrometry : RCM
- Publication Type :
- Academic Journal
- Accession number :
- 19283784
- Full Text :
- https://doi.org/10.1002/rcm.3981