CAP37-derived antimicrobial peptides have in vitro antiviral activity against adenovirus and herpes simplex virus type 1.

Authors :: Gordon YJ
Romanowski EG
Shanks RM
Yates KA
Hinsley H
Pereira HA
Source :: Current eye research [Curr Eye Res] 2009 Mar; Vol. 34 (3), pp. 241-9.
Publication Year :: 2009
Abstract: Purpose: The antiviral activity of an established antibacterial CAP37 domain and its extracellular mechanism of action were investigated.<br />Methods: CAP37-derived peptides modified to assess the importance of disulfide bonds were evaluated in cytotoxicity and antiviral assays (direct time kill, dose dependency, and TOTO-1) for adenovirus (Ad) and herpes simplex virus type 1 (HSV-1).<br />Results: Variable virus, adenovirus serotype-dependent, and dose-dependent inhibition were demonstrated without cytotoxicity. For peptide A (CAP37(20-44)), TOTO-1 dye uptake was demonstrated for Ad5 and HSV-1.<br />Conclusions: Unlike the antibacterial activity of this CAP37 domain, its antiviral activity is not fully dependent upon disulfide bond formation. Viral inhibition appears to result, in part, from disruption of the envelope and/or capsid.

Subjects :: Adenoviruses, Human physiology
Amino Acid Sequence
Antimicrobial Cationic Peptides chemistry
Antiviral Agents chemistry
Blood Proteins chemistry
Capsid drug effects
Carrier Proteins chemistry
Dose-Response Relationship, Drug
Herpesvirus 1, Human physiology
Molecular Sequence Data
Peptide Fragments chemistry
Structure-Activity Relationship
Adenoviruses, Human drug effects
Antimicrobial Cationic Peptides pharmacology
Antiviral Agents pharmacology
Blood Proteins pharmacology
Carrier Proteins pharmacology
Herpesvirus 1, Human drug effects
Peptide Fragments pharmacology

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