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[Functioning of tyrosine protein kinases and phosphatases in gastric mucosa cells under conditions of oxidative and nitrosative stress in gastric lesions].
- Source :
-
Ukrains'kyi biokhimichnyi zhurnal (1999 ) [Ukr Biokhim Zh (1999)] 2008 Sep-Oct; Vol. 80 (5), pp. 85-94. - Publication Year :
- 2008
-
Abstract
- Acute cold stress caused lesions of gastric mucosa as a result of its attack by active oxygen and nitrogen compounds. The tissue regeneration is regulated by a cascade of tyrosine protein kinases. Gastric ulceration leads to a decrease in activity of tyrosine protein kinases and phosphatases, following by fall in phosphotyrosine content in proteins of plasma membranes of gastric mucosa cells. No changes in superoxide dismutase activity, slight increase in catalase activity, inhibition of glutathione peroxydase, significant increase in OH* content and decrease in zinc level were observed in the gastric mucosa cells of stressed rats. That increased oxidative damage can lead to inactivation of protein tyrosine phosphatases. Nitric oxide synthase activity was three times higher in gastric mucosa cells after the cold stress. That can promote nitrosylation of tyrosine residues. During following days nitric oxide synthase activity remains high. Superoxide dismutase is activated on the 4 and 5th day after the stress. Catalase activity normalizes after second day. Tyrosine protein kinase activity increases in membranes with maximum on the 4th day, and remains inhibited in cytosole. Tyrosine protein phosphatases keep inhibited as well. Gluthatione peroxydase activity and zinc level decreased on the 5th day. Obtained results can be the evidence of violations in signal transduction through protein tyrosine kinase cascades, due to the reduction in tyrosine phosphorylation, as a result of increase in the content of active oxygen and nitrogen species.
- Subjects :
- Animals
Cell Membrane enzymology
Cell Membrane metabolism
Gastric Mucosa cytology
Gastric Mucosa enzymology
Hypothermia complications
Hypothermia enzymology
Hypothermia metabolism
Male
Rats
Reactive Oxygen Species metabolism
Restraint, Physical
Stomach Ulcer enzymology
Stomach Ulcer etiology
Stress, Psychological complications
Stress, Psychological enzymology
Stress, Psychological metabolism
Gastric Mucosa metabolism
Nitric Oxide metabolism
Oxidative Stress
Phosphoric Monoester Hydrolases metabolism
Protein-Tyrosine Kinases metabolism
Stomach Ulcer metabolism
Subjects
Details
- Language :
- Ukrainian
- Volume :
- 80
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Ukrains'kyi biokhimichnyi zhurnal (1999 )
- Publication Type :
- Academic Journal
- Accession number :
- 19248621