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Pigmentiphaga litoralis sp. nov., a facultatively anaerobic bacterium isolated from a tidal flat sediment.
- Source :
-
International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2009 Mar; Vol. 59 (Pt 3), pp. 521-5. - Publication Year :
- 2009
-
Abstract
- A novel Gram-negative, facultatively anaerobic, non-sporulating, non-motile, catalase- and oxidase-positive, rod-shaped bacterium (strain JSM 061001(T)) was isolated from a tidal flat in the South China Sea, China. Growth occurred with 0-5 % (w/v) NaCl [optimum, 0.5-1 % (w/v) NaCl], at pH 5.0-10.0 (optimum, pH 7.0) and at 4-35 degrees C (optimum, 25-30 degrees C). The major cellular fatty acids were C(16 : 0), cyclo C(17 : 0), C(18 : 1)omega7c and C(16 : 1). Strain JSM 061001(T) contained ubiquinone Q-8 as the predominant respiratory quinone, and phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid as the polar lipids. The genomic DNA G+C content was 65.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 061001(T) belongs to the family Alcaligenaceae and was related most closely to the type strains of the two recognized species of the genus Pigmentiphaga. The three strains formed a robust cluster in the neighbour-joining, maximum-parsimony and maximum-likelihood phylogenetic trees. Levels of DNA-DNA relatedness between strain JSM 061001(T) and the type strains of Pigmentiphaga daeguensis and Pigmentiphaga kullae were 15.8 and 10.5 %, respectively. The combination of phylogenetic analysis, DNA-DNA hybridization data, phenotypic characteristics and chemotaxonomic differences supported the view that strain JSM 061001(T) represents a novel species of the genus Pigmentiphaga, for which the name Pigmentiphaga litoralis sp. nov. is proposed. The type strain is JSM 061001(T) (=CCTCC AA207034(T)=KCTC 22165(T)).
- Subjects :
- Alcaligenaceae genetics
Alcaligenaceae isolation & purification
Alcaligenaceae physiology
Anaerobiosis
Bacterial Typing Techniques
Base Composition
China
DNA, Bacterial
DNA, Ribosomal
Genes, rRNA
Molecular Sequence Data
Nucleic Acid Hybridization
Phenotype
Phylogeny
RNA, Ribosomal, 16S
Sequence Analysis, DNA
Species Specificity
Alcaligenaceae classification
Geologic Sediments microbiology
Seawater microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1466-5026
- Volume :
- 59
- Issue :
- Pt 3
- Database :
- MEDLINE
- Journal :
- International journal of systematic and evolutionary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 19244433
- Full Text :
- https://doi.org/10.1099/ijs.0.002949-0