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Effect of progesterone on HLA-E gene expression in JEG-3 choriocarcinoma cell line.
- Source :
-
American journal of reproductive immunology (New York, N.Y. : 1989) [Am J Reprod Immunol] 2009 Mar; Vol. 61 (3), pp. 221-6. - Publication Year :
- 2009
-
Abstract
- Problem: Among class Ib human leukocyte antigen (HLA) molecules, HLA-E is known to be a major ligand of CD94/NKG2 receptor on natural killer (NK) cells, and to play a pivotal role in recognition of extravillous trophoblasts (EVTs) by maternal immune cells. However, it is scarcely known how HLA-E expression is regulated in EVTs.<br />Method of Study: In this study, we investigated whether progesterone, an essential hormone in maintaining pregnancy, regulated HLA-E expression in EVT-like cell line, JEG-3. HLA-E mRNA amount in cultured JEG-3 cells was assessed by real-time PCR and cell-surface HLA-E protein was analyzed by flowcytometry.<br />Results: Real-time PCR showed 3.5-fold increase 1 hour after the addition of 1000 ng/ml progesterone. This response was diminished by the addition of RU486, an antagonist for progesterone receptor. Flowcytometry indicated that 1000 ng/ml progesterone slightly enhanced HLA-E expression on the surface of JEG-3.<br />Conclusion: These results suggest that progesterone up-regulates HLA-E expression in JEG-3 cells through the pathway mediated by progesterone receptor. Our findings might give a new insight into immunomodulatory function of progesterone at fetomaternal interface.
- Subjects :
- Cell Line, Tumor
Choriocarcinoma
Gene Expression drug effects
Humans
Mifepristone pharmacology
Progesterone pharmacology
RNA, Messenger metabolism
Receptors, Progesterone drug effects
Trophoblasts drug effects
Up-Regulation drug effects
HLA-E Antigens
Gene Expression Regulation
HLA Antigens genetics
Histocompatibility Antigens Class I genetics
Progesterone physiology
Receptors, Progesterone physiology
Trophoblasts metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1600-0897
- Volume :
- 61
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- American journal of reproductive immunology (New York, N.Y. : 1989)
- Publication Type :
- Academic Journal
- Accession number :
- 19239424
- Full Text :
- https://doi.org/10.1111/j.1600-0897.2008.00684.x