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Biomaterial effects in articular cartilage tissue engineering using polyglycolic acid, a novel marine origin biomaterial, IGF-I, and TGF-beta 1.
- Source :
-
Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine [Proc Inst Mech Eng H] 2009 Jan; Vol. 223 (1), pp. 63-73. - Publication Year :
- 2009
-
Abstract
- Bovine articular chondrocytes were seeded on either polyglycolic acid (PGA) non-woven mesh scaffolds or a biomatrix from the species Porites lutea (POR). These constructs were cultured for 6 weeks in the presence of insulin-like growth factor (IGF)-I (10 ng/ml or 100 ng/ml) or transforming growth factor (TGF)-beta 1 (5 ng/ml or 30 ng/ml) to determine the in-vitro articular cartilage regeneration capacity of each. Histology, deoxyribonucleic acid content, collagen I and II (immunohistochemistry and enzyme-linked immunosorbent assay), and glycosaminoglycan (GAG) contents were measured at 0 weeks, 2 weeks, and 6 weeks to assess the characteristics of chondrogenesis. Both scaffolds supported the maintenance of the chondrocytic phenotype, as evidenced by the predominance of collagen II and the presence of rounded chondrocytes embedded in lacunae. Regardless of growth factor treatment, cells cultured on PGA scaffolds produced more collagen type II than those cultured on POR. Conversely, by 6 weeks, cells cultured on POR scaffolds produced more GAG than those cultured on PGA scaffolds, again regardless of the growth factor used. Across the two groups, 100 ng/ml of IGF-I had the greatest overall effect in GAG content. This work indicates that PGA and the POR scaffolds are both effective growth matrices for articular cartilage, with each scaffold exhibiting different yet desirable profiles of articular cartilage growth.
- Subjects :
- Animals
Biocompatible Materials chemistry
Biocompatible Materials pharmacology
Cartilage, Articular drug effects
Cattle
Cell Culture Techniques methods
Cell Differentiation drug effects
Cells, Cultured
Chondrocytes drug effects
Chondrocytes physiology
Extracellular Matrix chemistry
Polyglycolic Acid chemistry
Anthozoa chemistry
Cartilage, Articular cytology
Cartilage, Articular growth & development
Chondrocytes cytology
Extracellular Matrix metabolism
Insulin-Like Growth Factor I administration & dosage
Tissue Engineering methods
Transforming Growth Factor beta1 administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 0954-4119
- Volume :
- 223
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine
- Publication Type :
- Academic Journal
- Accession number :
- 19239068
- Full Text :
- https://doi.org/10.1243/09544119JEIM424