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[Effects of ligustrazine on extracellular acetylcholine levels in rat brain dialysate].
- Source :
-
Yao xue xue bao = Acta pharmaceutica Sinica [Yao Xue Xue Bao] 2008 Nov; Vol. 43 (11), pp. 1128-33. - Publication Year :
- 2008
-
Abstract
- Using brain microdialysis and LC-MS/MS to detect acetylcholine in rat brain to investigate the effects of ligustrazine. A liquid chromatography-tandem mass spectrometry method has been developed and validated for the determination of acetylcholine in rat brain dialysate sampling by microdialysis. The results indicated that ligustrazine administration by subcutaneous injection significantly increased Ach release in rat medial prefrontal cortex and nucleus accumbens in a dose-related manner. The drug' s effect on Ach release in rat brain could be directly detected by microdialysis combined with HPLC-MS/MS and this method is selective and sensitive.
- Subjects :
- Animals
Chromatography, High Pressure Liquid
Dose-Response Relationship, Drug
Ligusticum chemistry
Male
Microdialysis
Plants, Medicinal chemistry
Pyrazines administration & dosage
Pyrazines isolation & purification
Rats
Rats, Sprague-Dawley
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry
Acetylcholine metabolism
Nucleus Accumbens metabolism
Prefrontal Cortex metabolism
Pyrazines pharmacology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0513-4870
- Volume :
- 43
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Yao xue xue bao = Acta pharmaceutica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 19239033