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Gene expression profiling of peripheral blood in patients with abdominal aortic aneurysm.
- Source :
-
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery [Eur J Vasc Endovasc Surg] 2009 Jul; Vol. 38 (1), pp. 104-12. Date of Electronic Publication: 2009 Feb 23. - Publication Year :
- 2009
-
Abstract
- Object: Abdominal aortic aneurysm (AAA) pathogenesis remains poorly understood. This study investigated the gene expression profile of peripheral blood from patients with AAA using microarray technology.<br />Methods and Results: We determined gene expression profiles in pooled RNA from 10 AAA patients and 10 matched controls with arrays representing 14,000 transcripts. Microarray data for selected genes were confirmed by real-time PCR in two different AAA (n=36) and control (n=36) populations and integrated with biochemical data. We identified 91 genes which were differentially expressed in AAA patients. Gene Ontology analysis indicated a significant alteration of oxygen transport (increased hemoglobin gene expression) and lipid metabolism [including monoglyceride lipase and low density lipoprotein receptor-related protein 5 (LRP5) gene]. LRP5 expression was associated inversely with serum lipoprotein(a) [Lp(a)] concentration.<br />Conclusions: Increased expression of hemoglobin chain genes as well as of genes involved in erythrocyte mechanical stability were observed in the AAA RNA pools. The association between low levels of LRP5 gene expression and increased levels of Lp(a) in AAA patients suggests a potential role of LRP5 in Lp(a) catabolism. Our data underline the power of microarrays in identifying further molecular perturbations associated with AAA.
- Subjects :
- Aged
Aged, 80 and over
Aortic Aneurysm, Abdominal blood
Female
Humans
LDL-Receptor Related Proteins biosynthesis
Lipoprotein(a) biosynthesis
Low Density Lipoprotein Receptor-Related Protein-5
Male
Middle Aged
Monoacylglycerol Lipases biosynthesis
Polymerase Chain Reaction
Prognosis
Aortic Aneurysm, Abdominal genetics
DNA genetics
Gene Expression Profiling methods
Gene Expression Regulation
LDL-Receptor Related Proteins genetics
Lipoprotein(a) genetics
Monoacylglycerol Lipases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2165
- Volume :
- 38
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery
- Publication Type :
- Academic Journal
- Accession number :
- 19233690
- Full Text :
- https://doi.org/10.1016/j.ejvs.2009.01.020