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beta-Glucan administration enhances disease resistance and some innate immune responses in zebrafish (Danio rerio).
- Source :
-
Fish & shellfish immunology [Fish Shellfish Immunol] 2009 Aug; Vol. 27 (2), pp. 369-73. Date of Electronic Publication: 2009 Feb 14. - Publication Year :
- 2009
-
Abstract
- The present study was conducted to investigate the effect of beta-glucan (derived from Saccharomyces cerevisiae) on the immune response and its protection against an infection of the bacterial pathogen Aeromonas hydrophila in zebrafish (Danio rerio). Zebrafish received beta-glucan by intraperitoneal injection at three different concentrations (5, 2 and 0.5 mgml(-1)) at 6, 4 and 2 days prior the challenge. On challenge day the control and beta-glucan pretreated zebrafish were intraperitoneally injected with A. hydrophila and mortality was recorded for 4 days. Intraperitoneal injection of 5 mgml(-1) of beta-glucan significantly reduced the mortality. A single injection of 5 mgml(-1) of beta-glucan 6 days before challenge also enhanced significantly the survival against the infection. The treatment with beta-glucan increased the myelomonocytic cell population in the kidney at 6h postchallenge with A. hydrophila. Moreover it enhanced the ability of kidney cells to kill A. hydrophila. beta-glucan did not affect the expression of TNFalpha or IL-1 beta but seemed to modulate IFNgamma and chemokine expression in kidney.
- Subjects :
- Aeromonas hydrophila physiology
Animals
Cytokines blood
Fish Diseases mortality
Gene Expression Regulation drug effects
Gram-Negative Bacterial Infections immunology
Gram-Negative Bacterial Infections mortality
Kidney cytology
Survival Analysis
Adjuvants, Immunologic pharmacology
Fish Diseases immunology
Gram-Negative Bacterial Infections veterinary
Immunity, Innate drug effects
Zebrafish immunology
beta-Glucans pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9947
- Volume :
- 27
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Fish & shellfish immunology
- Publication Type :
- Academic Journal
- Accession number :
- 19232393
- Full Text :
- https://doi.org/10.1016/j.fsi.2009.02.007