Back to Search Start Over

beta-Glucan administration enhances disease resistance and some innate immune responses in zebrafish (Danio rerio).

Authors :
Rodríguez I
Chamorro R
Novoa B
Figueras A
Source :
Fish & shellfish immunology [Fish Shellfish Immunol] 2009 Aug; Vol. 27 (2), pp. 369-73. Date of Electronic Publication: 2009 Feb 14.
Publication Year :
2009

Abstract

The present study was conducted to investigate the effect of beta-glucan (derived from Saccharomyces cerevisiae) on the immune response and its protection against an infection of the bacterial pathogen Aeromonas hydrophila in zebrafish (Danio rerio). Zebrafish received beta-glucan by intraperitoneal injection at three different concentrations (5, 2 and 0.5 mgml(-1)) at 6, 4 and 2 days prior the challenge. On challenge day the control and beta-glucan pretreated zebrafish were intraperitoneally injected with A. hydrophila and mortality was recorded for 4 days. Intraperitoneal injection of 5 mgml(-1) of beta-glucan significantly reduced the mortality. A single injection of 5 mgml(-1) of beta-glucan 6 days before challenge also enhanced significantly the survival against the infection. The treatment with beta-glucan increased the myelomonocytic cell population in the kidney at 6h postchallenge with A. hydrophila. Moreover it enhanced the ability of kidney cells to kill A. hydrophila. beta-glucan did not affect the expression of TNFalpha or IL-1 beta but seemed to modulate IFNgamma and chemokine expression in kidney.

Details

Language :
English
ISSN :
1095-9947
Volume :
27
Issue :
2
Database :
MEDLINE
Journal :
Fish & shellfish immunology
Publication Type :
Academic Journal
Accession number :
19232393
Full Text :
https://doi.org/10.1016/j.fsi.2009.02.007