Back to Search
Start Over
Phenotypic differences between BCG vaccines at the proteome level.
- Source :
-
Tuberculosis (Edinburgh, Scotland) [Tuberculosis (Edinb)] 2009 Mar; Vol. 89 (2), pp. 126-35. Date of Electronic Publication: 2009 Feb 20. - Publication Year :
- 2009
-
Abstract
- To contribute to Mycobacterium bovis BCG characterization, two substrains were analyzed using two-dimensional gel electrophoresis (2D-PAGE) and mass spectrometry (MS), based on their protective efficacy in a pulmonary-tuberculosis mouse model. Cell-fraction proteins of BCG Denmark and Phipps substrains were separated into approximately 500 spots in 2D-PAGE. The proteomes were similar in protein number, and isoelectric point (pI) and molecular mass (MM) distribution. Statistical analysis, resulted in 72 spots with no change, and 168 and 90 unique for BCG Phipps or Denmark, respectively. Two hundred and fourteen spots showed changes in intensity of >1-fold, 138 of Denmark, and 76 of Phipps. Seventeen spots were selected for MS-based identification (13 from Phipps and 4 from Denmark), including unique, as well as proteins with changes in intensity. The proteins identified participate in virulence, detoxification, adaptation, lipid metabolism, information pathways, cell wall and cell processes, intermediary metabolism and respiration, or still hypotheticals. Our findings contribute to phenotype characterization of BCG substrains and provide new elements to consider for the design of diagnostic tools, drug targets and a new vaccine against tuberculosis based upon protein expression through quantitative statistical analysis.
- Subjects :
- Animals
BCG Vaccine classification
Bacterial Proteins analysis
Bacterial Proteins genetics
Bacterial Proteins physiology
Electrophoresis, Gel, Two-Dimensional methods
Humans
Mice
Mycobacterium bovis chemistry
Mycobacterium bovis genetics
Proteomics methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods
BCG Vaccine chemistry
Mycobacterium bovis classification
Proteome classification
Subjects
Details
- Language :
- English
- ISSN :
- 1873-281X
- Volume :
- 89
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Tuberculosis (Edinburgh, Scotland)
- Publication Type :
- Academic Journal
- Accession number :
- 19231290
- Full Text :
- https://doi.org/10.1016/j.tube.2008.12.001