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Reassessment of the role of FKBP38 in the Rheb/mTORC1 pathway.
- Source :
-
FEBS letters [FEBS Lett] 2009 Mar 18; Vol. 583 (6), pp. 965-70. Date of Electronic Publication: 2009 Feb 15. - Publication Year :
- 2009
-
Abstract
- The small G-protein Rheb regulates cell growth via the mTORC1 complex by incompletely understood mechanisms. Recent studies document that Rheb activates mTORC1 via direct, GTP-dependent interaction with the peptidyl-prolyl-cis/trans-isomerase FKBP38, which is proposed to act as an inhibitor of mTORC1. We have conducted a comprehensive biochemical characterization of the Rheb/FKBP38 interaction. Using three different in vitro assays we did not detect an interaction between Rheb and FKBP38. Cell biological experiments illustrate that FKBP38 plays only a very minor, if any, role in mTORC1 activation. Our data document that FKBP38 is not the long-sought Rheb effector linking Rheb to mTORC1 activation.
- Subjects :
- Cells, Cultured
Humans
Insulin pharmacology
Mechanistic Target of Rapamycin Complex 1
Monomeric GTP-Binding Proteins genetics
Monomeric GTP-Binding Proteins metabolism
Multiprotein Complexes
Neuropeptides genetics
Neuropeptides metabolism
Phosphorylation drug effects
Protein Binding
Proteins
RNA, Small Interfering pharmacology
Ras Homolog Enriched in Brain Protein
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins metabolism
Recombinant Fusion Proteins physiology
Ribosomal Protein S6 Kinases metabolism
Signal Transduction drug effects
Signal Transduction genetics
Signal Transduction physiology
TOR Serine-Threonine Kinases
Tacrolimus Binding Proteins antagonists & inhibitors
Tacrolimus Binding Proteins genetics
Transcription Factors metabolism
Transcriptional Activation drug effects
Transfection
Monomeric GTP-Binding Proteins physiology
Neuropeptides physiology
Tacrolimus Binding Proteins physiology
Transcription Factors physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3468
- Volume :
- 583
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 19222999
- Full Text :
- https://doi.org/10.1016/j.febslet.2009.02.015