Back to Search
Start Over
Immunocytochemical study of granular duct cells with a hormonally enhanced granular cell phenotype in the mouse parotid gland.
- Source :
-
Odontology [Odontology] 2009 Jan; Vol. 97 (1), pp. 57-61. Date of Electronic Publication: 2009 Jan 29. - Publication Year :
- 2009
-
Abstract
- In the parotid glands (PGs) of intact male mice (12 weeks of age, ICR strain), immunofluorescence labels for a true tissue kallikrein, mK1, and for nerve growth factor (NGF) were recognized through the subluminal edges of the striated duct (SD) segments and interlobular duct segments. Because of their small size, secretory granules were not detectable by light microscopy in any of the duct cells. Full-fledged granular cells, containing large secretory granules that were visible by light microscopy, were induced in the SD segments of male mice after the injection of 5alpha-dehydrotestosterone (DHT) and triiodothyronine (T(3)), given either alone or in combination every other day for 2 weeks. A stronger effect was detected in the mice that were concomitantly injected with DHT and T(3), and more abundant, fully developed granular cells appeared in the SD segments of these mice. These full-fledged granular cells were immunoreactive for mK1, NGF, and epidermal growth factor, but not for renin. The present results indicate that some of the SD cells with small granules in the mouse PG can develop a granular cell phenotype, producing more kinds of growth factors, as a result of the actions of androgen and thyroid hormone.
- Subjects :
- Animals
Dihydrotestosterone pharmacology
Epidermal Growth Factor analysis
Epidermal Growth Factor drug effects
Fluorescent Antibody Technique, Indirect
Immunohistochemistry
Male
Mice
Mice, Inbred ICR
Nerve Growth Factor analysis
Nerve Growth Factor drug effects
Parotid Gland drug effects
Phenotype
Renin analysis
Salivary Ducts drug effects
Secretory Vesicles drug effects
Secretory Vesicles ultrastructure
Tissue Kallikreins analysis
Tissue Kallikreins drug effects
Triiodothyronine pharmacology
Parotid Gland cytology
Salivary Ducts cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1618-1247
- Volume :
- 97
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Odontology
- Publication Type :
- Academic Journal
- Accession number :
- 19184300
- Full Text :
- https://doi.org/10.1007/s10266-008-0090-3