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Degradation of transglutaminase 2 by calcium-mediated ubiquitination responding to high oxidative stress.
- Source :
-
FEBS letters [FEBS Lett] 2009 Feb 18; Vol. 583 (4), pp. 648-54. Date of Electronic Publication: 2009 Feb 01. - Publication Year :
- 2009
-
Abstract
- Transglutaminase 2 (TG2) is a calcium-dependent enzyme that catalyzes the transamidation reaction. There is conflicting evidence on the role of TG2 in apoptosis. In this report, we show that TG2 increases in response to low level of oxidative stress, whereas TG2 diminishes under high stress conditions. Monitoring TG2 expression, activity and calcium concentration in cells treated with A23187 revealed that the initial rise of calcium activates TG2 but subsequent calcium-overload induces the degradation of TG2 via calcium-mediated polyubiquitination. These results indicate that the role of TG2 in apoptosis depends on the level of calcium influx triggered by oxidative stress.
- Subjects :
- Adenoviridae genetics
Anti-Bacterial Agents pharmacology
Apoptosis
Calcimycin pharmacology
Cell Death
Cell Line
Culture Media, Serum-Free
Dose-Response Relationship, Drug
Enzyme Inhibitors pharmacology
HeLa Cells
Humans
Kidney cytology
Leupeptins pharmacology
Protein Glutamine gamma Glutamyltransferase 2
Time Factors
Ubiquitination
Calcium metabolism
GTP-Binding Proteins metabolism
Oxidative Stress
Transglutaminases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3468
- Volume :
- 583
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 19183553
- Full Text :
- https://doi.org/10.1016/j.febslet.2009.01.032