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Transcriptome-guided characterization of genomic rearrangements in a breast cancer cell line.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2009 Feb 10; Vol. 106 (6), pp. 1886-91. Date of Electronic Publication: 2009 Jan 30. - Publication Year :
- 2009
-
Abstract
- We have identified new genomic alterations in the breast cancer cell line HCC1954, using high-throughput transcriptome sequencing. With 120 Mb of cDNA sequences, we were able to identify genomic rearrangement events leading to fusions or truncations of genes including MRE11 and NSD1, genes already implicated in oncogenesis, and 7 rearrangements involving other additional genes. This approach demonstrates that high-throughput transcriptome sequencing is an effective strategy for the characterization of genomic rearrangements in cancers.
- Subjects :
- Base Sequence
Carrier Proteins genetics
Cell Line, Tumor
DNA, Complementary
DNA-Binding Proteins genetics
Female
Histone-Lysine N-Methyltransferase
Humans
MRE11 Homologue Protein
Neoplasm Proteins genetics
Nuclear Proteins genetics
Breast Neoplasms genetics
Gene Expression Profiling methods
Gene Rearrangement
Genome, Human genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1091-6490
- Volume :
- 106
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 19181860
- Full Text :
- https://doi.org/10.1073/pnas.0812945106