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Polar localization of virulence-related Esx-1 secretion in mycobacteria.
- Source :
-
PLoS pathogens [PLoS Pathog] 2009 Jan; Vol. 5 (1), pp. e1000285. Date of Electronic Publication: 2009 Jan 30. - Publication Year :
- 2009
-
Abstract
- The Esx-1 (type VII) secretion system is critical for virulence of both Mycobacterium tuberculosis and Mycobacterium marinum, and is highly conserved between the two species. Despite its importance, there has been no direct visualization of Esx-1 secretion until now. In M. marinum, we show that secretion of Mh3864, a novel Esx-1 substrate that remains partially cell wall-associated after translocation, occurred in polar regions, indicating that Esx-1 secretion takes place in these regions. Analysis of Esx-1 secretion in infected host cells suggested that Esx-1 activity is similarly localized in vivo. A core component of the Esx-1 apparatus, Mh3870, also localized to bacterial poles, showing a preference for new poles with active cell wall peptidoglycan (PGN) synthesis. This work demonstrates that the Esx-1 secretion machine localizes to, and is active at, the bacterial poles. Thus, virulence-related protein secretion is localized in mycobacteria, suggesting new potential therapeutic targets, which are urgently needed.
- Subjects :
- 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase metabolism
Actins
Animals
Antigens, Bacterial genetics
Bacterial Proteins genetics
Cell Line
Cell Polarity
Cell Wall metabolism
Mice
Mutagenesis
Mycobacterium marinum genetics
Peptidoglycan metabolism
Virulence Factors genetics
Antigens, Bacterial metabolism
Bacterial Proteins metabolism
Mycobacterium marinum metabolism
Virulence Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1553-7374
- Volume :
- 5
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- PLoS pathogens
- Publication Type :
- Academic Journal
- Accession number :
- 19180234
- Full Text :
- https://doi.org/10.1371/journal.ppat.1000285