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Expression of two human acyl-CoA:diacylglycerol acyltransferase isozymes in yeast and selectivity of microbial inhibitors toward the isozymes.
- Source :
-
The Journal of antibiotics [J Antibiot (Tokyo)] 2009 Jan; Vol. 62 (1), pp. 51-4. Date of Electronic Publication: 2009 Jan 09. - Publication Year :
- 2009
-
Abstract
- Two isozymes for human acyl-coenzyme A:diacylglycerol acyltransferase (DGAT), DGAT1 and DGAT2, were independently expressed in DGAT-deficient Saccharomyces cerevisiae to establish DGAT1- and DGAT2-S. cerevisiae. The selectivity of DGAT inhibitors of natural origin towards the isozymes was assessed in enzyme assays using the microsomal fractions prepared from DGAT1- and DGAT2-S. cerevisiae. Amidepsines and xanthohumol inhibited DGAT1 and DGAT2 with similar potency, whereas roselipins were found to inhibit DGAT2 selectively.
- Subjects :
- Animals
DNA, Complementary genetics
Diacylglycerol O-Acyltransferase genetics
Enzyme Inhibitors chemistry
Humans
Isoenzymes antagonists & inhibitors
Isoenzymes biosynthesis
Liver enzymology
Molecular Sequence Data
Plasmids genetics
Rats
Reverse Transcriptase Polymerase Chain Reaction
Diacylglycerol O-Acyltransferase antagonists & inhibitors
Diacylglycerol O-Acyltransferase biosynthesis
Enzyme Inhibitors pharmacology
Saccharomyces cerevisiae enzymology
Saccharomyces cerevisiae genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1881-1469
- Volume :
- 62
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Journal of antibiotics
- Publication Type :
- Academic Journal
- Accession number :
- 19132064
- Full Text :
- https://doi.org/10.1038/ja.2008.5