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S1P/S1P1 signaling stimulates cell migration and invasion in Wilms tumor.
- Source :
-
Cancer letters [Cancer Lett] 2009 Apr 18; Vol. 276 (2), pp. 171-9. Date of Electronic Publication: 2009 Jan 07. - Publication Year :
- 2009
-
Abstract
- Sphingosine-1-phosphate (S1P) is an important regulator of cellular functions via interaction with its receptors S1P(1-5). To date, nothing is known about the S1P receptor expression and the effects of S1P signaling in Wilms tumor. In this study, we found ubiquitous expression of S1P receptors in Wilms tumor specimens and cell lines. We demonstrated that S1P(1) acted as a promigratory modulator by employing S1P(1) antagonist VPC44116, S1P(1) siRNA and adenoviral transduction in Wilms tumor cells. Further, we clarified that S1P(1)-mediated migration occurred via Gi coupling and activation of PI3K and Rac1. In addition, S1P stimulated WiT49 cell invasion through S1P(1)/Gi signaling pathway. We consider that targeting S1P(1) may be a point of therapeutic intervention in Wilms tumor.
- Subjects :
- Cell Line, Tumor
Cell Movement
GTP-Binding Protein alpha Subunits, Gi-Go physiology
Humans
Neoplasm Invasiveness
Phosphatidylinositol 3-Kinases physiology
RNA, Small Interfering pharmacology
Receptors, Lysosphingolipid analysis
Receptors, Lysosphingolipid antagonists & inhibitors
Receptors, Lysosphingolipid physiology
Sphingosine physiology
rac1 GTP-Binding Protein physiology
Kidney Neoplasms pathology
Lysophospholipids physiology
Signal Transduction physiology
Sphingosine analogs & derivatives
Wilms Tumor pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7980
- Volume :
- 276
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Cancer letters
- Publication Type :
- Academic Journal
- Accession number :
- 19131156
- Full Text :
- https://doi.org/10.1016/j.canlet.2008.11.025