A selective inhibitor Gal-PUGNAc of human lysosomal beta-hexosaminidases modulates levels of the ganglioside GM2 in neuroblastoma cells.

Authors :: Stubbs KA
Macauley MS
Vocadlo DJ
Source :: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2009; Vol. 48 (7), pp. 1300-3.
Publication Year :: 2009
Abstract: Gal-PUGNAc (see picture), a highly selective inhibitor for beta-hexosaminidases HEXA and HEXB is cell-permeable and modulates the activity of HEXA and HEXB in tissue culture, increasing ganglioside GM2 levels. Gal-PUGNAc should allow the role of these enzymes to be studied at the cellular level without generating a complex chemical phenotype from concomitant inhibition of O-GlcNAcase.

Subjects :: Cell Line, Tumor
Enzyme Inhibitors chemistry
Enzyme Inhibitors metabolism
G(M2) Ganglioside chemistry
Hexosaminidase A antagonists & inhibitors
Hexosaminidase A metabolism
Hexosaminidase B antagonists & inhibitors
Hexosaminidase B metabolism
Humans
Lysosomes enzymology
Substrate Specificity
G(M2) Ganglioside metabolism
Neuroblastoma enzymology
beta-N-Acetylhexosaminidases antagonists & inhibitors
beta-N-Acetylhexosaminidases metabolism

Full Text Access

Tools