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Regulation of connexin gene expression during skeletal muscle regeneration in the adult rat.
- Source :
-
American journal of physiology. Cell physiology [Am J Physiol Cell Physiol] 2009 Mar; Vol. 296 (3), pp. C593-606. Date of Electronic Publication: 2009 Jan 07. - Publication Year :
- 2009
-
Abstract
- In the adult skeletal muscle, various kinds of trauma promote proliferation of satellite cells that differentiate into myoblasts forming new myofibers or to repair the damaged one. The aim of present work was to perform a comparative spatial and temporal analysis of connexin (Cx) 37, Cx39, Cx40, Cx43, and Cx45 expression in the adult regenerating skeletal muscle in response to crush injury. Within 24 h from injury, Cx37 expression was upregulated in the endothelial cells of blood vessels, and, 5 days after injury, Cx37-expressing cells were found inside the area of lesion and formed clusters generating new blood vessels with endothelial cells expressing Cx37. Three days after injury, Cx39 mRNA was selectively expressed in myogenin-positive cells, forming rows of closely apposed cell nuclei fusing in myotubes. Cx40 mRNA-labeled cells were observed within 24 h from injury in the endothelium of blood vessels, and, 5 days after lesion, Cx40-labeled cells were found inside the area of lesion-forming rows of myogenin-positive, closely apposed cells coexpressing Cx39. Within 24 h from lesion, both Cx43 and Cx45 mRNAs were upregulated in individual cells, and some of them were positive for M-cadherin. Three days after injury, a large number of both Cx43 and Cx45 mRNA-labeled and myogenin-positive cells were found inside the area of lesion. Taken together, these results show that at least four Cxs, out of five expressed in regenerating skeletal muscle, can be differentially involved in communication of myogenic cells during the process of cell proliferation, aggregation, and fusion to form new myotubes or to repair damaged myofibers.
- Subjects :
- Animals
Cell Aggregation
Cell Fusion
Cell Proliferation
Connexin 30
Connexin 43 metabolism
Connexins genetics
Constriction
Endothelial Cells metabolism
Gene Expression Regulation
Immunohistochemistry
In Situ Hybridization
Male
Muscle Fibers, Skeletal metabolism
Muscle, Skeletal blood supply
Muscle, Skeletal pathology
Muscle, Skeletal surgery
Neovascularization, Physiologic
RNA, Messenger metabolism
Rats
Rats, Wistar
Time Factors
Gap Junction alpha-5 Protein
Gap Junction alpha-4 Protein
Connexins metabolism
Muscle, Skeletal metabolism
Regeneration genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0363-6143
- Volume :
- 296
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Cell physiology
- Publication Type :
- Academic Journal
- Accession number :
- 19129462
- Full Text :
- https://doi.org/10.1152/ajpcell.00458.2008